Coding

Part:BBa_K1891003:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


N-Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 805


Design Notes

To get this part, we designed primers according to the gene sequence and then cloned it to our expression vector. Because it doesn't have a initiation codon，we added a initiation codon in the forward primer.

Source

We get this gene from pCambia1300-N-Luciferase plasmid.

References